BNRF Instruments

BioNano Research Facilities include laboratories on the Evansdale and Downtown campuses. BNRF houses instrumentation to help characterize and analyze the interaction of fabricated materials and their effect on mammalian cell cultures.

Click on the categories below to jump to those sections. See the SRF instrumentation list for contact and  location information.

Cell Culture

CO ₂ Incubators

• 4 -Thermo Scientific Heracell 150i CO2 Incubators
• dual chamber copper interior
• 5.3 cu.ft.
• CO ₂ regulator and gas guard

UV-Vis Microplate Spectrophotometer

BioTek Epoch spectrophotometer

• monochromator based UV-Vis 200nm to 999nm for absorbance readings
• no heating or shaking capabilities
• Take3 microvolume plate compatibility for DNA
  

Labconco FreeZone 4.5 Lyophilizer

Centrifugation

SORVALL Legend X1R

• General purpose refrigerated centrifuges
• Temperature control -10 °C to +40 C
• TX-400 swinging bucket rotors (max speed 5000rpm)
• 16×50mL or 36×15mL conical tube capacity
  
• Fiberlite 15 fixed angle rotor (max speed 15,000rpm)
  6×85mL round bottom or 6×50mL conical tubes
  This rotor only available at Engineering Sciences Building location

SORVALL Legend Micro 17R

• General purpose refrigerated microcentrifuges
• Temperature control -9 °Cto +40 °C
• 24×1.5/2.0mL rotor with bio-containment prevention lid
• Max speed 13,300 rpm

Microscopy

Brightfield Optical Microscope

AMG Micromaster inverted microscope

• Binocular body
• 4x, 10x, 20x objectives
• Brightfield

AMG EVOS CORE XL digital inverted microscope

• LED for transmitted light
• 4x, 10x, 20x, 40x objectives
• Brightfield & phase contrast
• 2 USB ports

Confocal Fluorescence Microscope

Olympus Spinning Disc Fluorescent Confocal Microscope 
Confocal Microscope SOP

• DSU spinning disc confocal system

• DAPI, FTIC,CY3, U/B/G fluorescence filter cubes

• CCD camera for high speed imaging and video recording

• 4×, 20×, 40× and 100× objectives lenses

• Motorized stage for 3D confocal imaging
  

Digital Inverted Fluorescent Microscope

• LED light cubes: GFP, RFP, YFP, DAPI
• 4x, 10x, 20x, 40x, 100x objectives
• Monochrome camera 1360×1024
• 3 USB, 1 DVI ports

Inverted Fluorescent Microscope

• Fluorescent filters: GFP, RFP, CY5, DAPI
• DIC, Brightfield and polarizers
• 4x, 10x, 20x, 40x, 63x objectives
• Monochrome camera 1392×1024

Synchronized Video Fluorescent Microscope

LABSMITH SVM340

• Synchronous pulsed fluorescence illuminator
• Camera module with 600-line resolution B&W camera
• 10X semi-plan DIN objective

LABSMITH HVS448

• High voltage sequencer
• 8 channels supplying/sensing up to 3.0kV
• 1kHz sequencing for microfluidics and MEMS

Spectroscopy

Fluorescence Spectroscopy

HORIBA JOBIN YVON FLUOROLOG-3 SPECTROFLUOROMETER

• Steady state wavelength measurements 230nm to 1550nm
• Lifetime wavelength measurements 250nm to 900nm (200psec to 100s of msec)
• TCSPC
• Temperature bath for -25 to 150 ºC
• Cuvette, thin film, multiwell plate sample holders

CD Spectropolarimeter

JASCO J-810 SPECTROPOLARIMETER

• Equipped with peltier temperature control (-10 to 110 °C)
• Measurement range: 163-900 nm
• Consist of a variable wavelength ploarimeter and absorption spectrophotometer
• Operated by Jasco’s Spectra Manager software

Mass Spectroscopy

Quadrupole and Orbitrap LC-MS/MS

Thermo Fisher Scientific Q-Exactive

• High-performance quadrupole precursor selection with high resolution, accurate-mass (HR/AM) Orbitrap detection
• Accela UHPLC for high-flow applications
• Easy-nLC 1000 UHPLC for nano-flow applications
• HESI & Nanospray flex ion source
• Sieve and Proteome Discoverer analytical software
• Peak nitrogen generator

SPEEDVAC

THERMO SCIENTIFIC SPEEDVAC

Savant SPD 111 P1

• Concentrator (standard evaporation)
• For solvents: acetonitrile, ethanol, isopropanol, methanol, water

chromatography

Thermo Fisher Scientific Accela Ultra High Performance Liquid Chromatography

• High resolution and high efficiency separations at reduced analysis times
• Photodiode array detector for absorbance measurements across multiple wavelengths 

Thermofisher Scientitic Easy-nano-Ultra High Performance Liquid Chromatography

Refrigeration

20.3 cu.ft. Thermo Scientific general lab refrigerator

• Temperature range 1 °C to 10 °C
• Manual defrost

Availability

• 1 in G75E Engineering Sciences Building
• 1 in 381 Chemistry Research Laboratory Building

Freezers

THERMO SCIENTIFIC LAB FREEZER

• 20.6 cu. ft.
• Temperature range -12 °C to -20 °C
• Manual defrost

THERMO SCIENTIFIC ULTRA LOW LAB FREEZER

• 24.4 cu. ft.
• Temperature -86 °C

Ice Maker

SCOTSMAN FLAKE ICE MAKER

• 80 lb. storage capacity, air cooled
• IceAssure water filtration system

Sterilization

Autoclaves

STERIS 250

• 20×20x38” chamber size

Heidolph Tuttnauer 3850 EP

• Tabletop autoclave
• 65L capacity, 15” diameter chamber

Electrophoresis

PROTEAN i12 IEF system

• Run 1-12 IPG strips in 7, 11, 13, 17, 18, or 24 cm
• Each channel in the isoelectric focusing tray is independently powered
• Each channel could run different sample types, pH gradients, and programs independently

ELECTROPHORESIS CHEMBERS (SDS-PAGE)

• Mini-Protean Tetra Vertical Eletrophoresis Cell, run 1 to 4 gels, Precast: 8.6 cm x 6.8 cm; handcast: 8.3 cm x 7.3 cm
• Mini-Protean Tetra Handcast system, for 1.0 mm thick and 10-well gel
• Criterion cell, run 1-2 gels, 13.3 cm x 8.7 cm
• Protean II XL cell, run 1-4 handcast gels, 18.5×20 cm

BLOTTING SYSTEM

• Mini-Protean Tetra Trans-Blot Module
• Criterion Blotter

CHEMIDOC MP IMAGER

The ChemiDoc MP system is a full-feature instrument for gel or western blot imaging.

• Used for fluorescent western blotting, chemiluminescence detection, and general gel documentation
• Installed Epi-white light and trans-UV (302 nm); trans-white conversion screen, and epi-red, epi-green, and epi-blue LED modules
• Image resolution: 4 megapixels
• Image Lab™ software for gel image acquisition
• PDQuest for 2D gel analysis